Coding

Part:BBa_K4601001

Designed by: Doriane Blaise   Group: iGEM23_Evry-Paris-Saclay   (2023-09-23)


ChrimsonR

This part is the E. coli codon optimised version of ChrimsonR channelrhodopsin.

Usage and Biology

ChrimsonR is a channelrhodopsin derived from the CR Chrimson of 'Chlamydomonas noctigama' that is naturally red-shifted [1]. Compared to the Chrimson, ChrimsonR contains one point mutation K176R that does not modify the absorption properties, but increases the photocurrent generated upon light irradiation. ChrimsonR is a Na+ inward pump that is also capable of transporting H+, K+ and Ca2+. It is one of the few microbial opsins in clinical trials for gene therapy for patients with documented diagnosis of non-syndromic Retinitis Pigmentosa [2].

For the characterisation of this part an expression cassette was assembled and the results are presented on its page in Parts Registry: BBa_K4601201.

References

[1] Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK, Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, Constantine-Paton M, Wong GK-S, Boyden ES. Independent optical excitation of distinct neural populations. Nature Methods (2014) 11: 338–346.

[2] GenSight Biologics. A Phase 1/2a, Open-label, non-randomized, dose-eEscalation study to evaluate the safety and tolerability of GS030 in subjects with Retinitis Pigmentosa. clinicaltrials.gov (2022).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None